DNA

Part:BBa_K3860009:Design

Designed by: Marius Schnutenhaus   Group: iGEM21_RUBochum   (2021-09-30)


Acetyl carborxylases complex from e. coli k12


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3562
    Illegal PstI site found at 2671
    Illegal PstI site found at 2713
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3562
    Illegal PstI site found at 2671
    Illegal PstI site found at 2713
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3562
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3562
    Illegal PstI site found at 2671
    Illegal PstI site found at 2713
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3562
    Illegal PstI site found at 2671
    Illegal PstI site found at 2713
    Illegal AgeI site found at 579
    Illegal AgeI site found at 1404
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 517
    Illegal SapI.rc site found at 714


Design Notes

The Acetyl-coA carboxylase complex was isolated from e. coli K12 and conneceted with glycin linkers.

Source

E. coli K12

References

https://www.ncbi.nlm.nih.gov/protein/16128178

https://www.ncbi.nlm.nih.gov/protein/16131143

https://www.ncbi.nlm.nih.gov/protein/16131144

https://www.ncbi.nlm.nih.gov/protein/CAD6008109.1