DNA
Part:BBa_K3860009:Design
Designed by: Marius Schnutenhaus Group: iGEM21_RUBochum (2021-09-30)
Acetyl carborxylases complex from e. coli k12
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3562
Illegal PstI site found at 2671
Illegal PstI site found at 2713 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3562
Illegal PstI site found at 2671
Illegal PstI site found at 2713 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3562
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3562
Illegal PstI site found at 2671
Illegal PstI site found at 2713 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3562
Illegal PstI site found at 2671
Illegal PstI site found at 2713
Illegal AgeI site found at 579
Illegal AgeI site found at 1404 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 517
Illegal SapI.rc site found at 714
Design Notes
The Acetyl-coA carboxylase complex was isolated from e. coli K12 and conneceted with glycin linkers.
Source
E. coli K12
References
https://www.ncbi.nlm.nih.gov/protein/16128178
https://www.ncbi.nlm.nih.gov/protein/16131143